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(A) Flow cytometry results of Caki-1 cells that were cocultured with GFP-histone H2B primary T cells. Data shows the percentage of GFP-H2B + Caki-1 cells in coculture relative to monocultured Caki-1 cells. (B) Mean fluorescent intensity of GFP-H2B expression of cocultured Caki-1 cells compared to monoculture (p value = 0.0058, unpaired T test was used to analyze the data). (C) Flow cytometry analysis of RCC cell lines cocultured with EdU-labeled primary human T cells. Plots show the percentage of RCC cells that are EdU + and CD45 + post 16-hour coculture. (D) Max intensity projection of Z-stacked images taken from a 10:1 coculture of EdU-labeled primary human T cells with ACHN cells. Left panels depict an enhanced view of an EdU + CD45 + trogosome within the intracellular space of an ACHN cell. Right panels depict an enhanced view of an EdU + CD45 + T cell for comparison purposes. (E) Representative max intensity projection images of trogosomes in three RCC cell lines generated from Z-stacks (top to bottom, A498, 786O, ACHN). Boxes in the merged images of middle and bottom panels indicate the region being enhanced in the split color panels to the right of the merged image. Arrows in the merged zoom panels indicate the location of a trogosome. All images taken on a Zeiss <t>710</t> laser scanning confocal, <t>40X</t> (NA 1.30).
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(A) Flow cytometry results of Caki-1 cells that were cocultured with GFP-histone H2B primary T cells. Data shows the percentage of GFP-H2B + Caki-1 cells in coculture relative to monocultured Caki-1 cells. (B) Mean fluorescent intensity of GFP-H2B expression of cocultured Caki-1 cells compared to monoculture (p value = 0.0058, unpaired T test was used to analyze the data). (C) Flow cytometry analysis of RCC cell lines cocultured with EdU-labeled primary human T cells. Plots show the percentage of RCC cells that are EdU + and CD45 + post 16-hour coculture. (D) Max intensity projection of Z-stacked images taken from a 10:1 coculture of EdU-labeled primary human T cells with ACHN cells. Left panels depict an enhanced view of an EdU + CD45 + trogosome within the intracellular space of an ACHN cell. Right panels depict an enhanced view of an EdU + CD45 + T cell for comparison purposes. (E) Representative max intensity projection images of trogosomes in three RCC cell lines generated from Z-stacks (top to bottom, A498, 786O, ACHN). Boxes in the merged images of middle and bottom panels indicate the region being enhanced in the split color panels to the right of the merged image. Arrows in the merged zoom panels indicate the location of a trogosome. All images taken on a Zeiss <t>710</t> laser scanning confocal, <t>40X</t> (NA 1.30).
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Carl Zeiss laser scanning confocal microscope zeiss lsm 880 axioexaminer.z1
(A) Flow cytometry results of Caki-1 cells that were cocultured with GFP-histone H2B primary T cells. Data shows the percentage of GFP-H2B + Caki-1 cells in coculture relative to monocultured Caki-1 cells. (B) Mean fluorescent intensity of GFP-H2B expression of cocultured Caki-1 cells compared to monoculture (p value = 0.0058, unpaired T test was used to analyze the data). (C) Flow cytometry analysis of RCC cell lines cocultured with EdU-labeled primary human T cells. Plots show the percentage of RCC cells that are EdU + and CD45 + post 16-hour coculture. (D) Max intensity projection of Z-stacked images taken from a 10:1 coculture of EdU-labeled primary human T cells with ACHN cells. Left panels depict an enhanced view of an EdU + CD45 + trogosome within the intracellular space of an ACHN cell. Right panels depict an enhanced view of an EdU + CD45 + T cell for comparison purposes. (E) Representative max intensity projection images of trogosomes in three RCC cell lines generated from Z-stacks (top to bottom, A498, 786O, ACHN). Boxes in the merged images of middle and bottom panels indicate the region being enhanced in the split color panels to the right of the merged image. Arrows in the merged zoom panels indicate the location of a trogosome. All images taken on a Zeiss <t>710</t> laser scanning confocal, <t>40X</t> (NA 1.30).
Laser Scanning Confocal Microscope Zeiss Lsm 880 Axioexaminer.Z1, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Carl Zeiss 100x, na 1.30 plan-neofluor lens
(A) Flow cytometry results of Caki-1 cells that were cocultured with GFP-histone H2B primary T cells. Data shows the percentage of GFP-H2B + Caki-1 cells in coculture relative to monocultured Caki-1 cells. (B) Mean fluorescent intensity of GFP-H2B expression of cocultured Caki-1 cells compared to monoculture (p value = 0.0058, unpaired T test was used to analyze the data). (C) Flow cytometry analysis of RCC cell lines cocultured with EdU-labeled primary human T cells. Plots show the percentage of RCC cells that are EdU + and CD45 + post 16-hour coculture. (D) Max intensity projection of Z-stacked images taken from a 10:1 coculture of EdU-labeled primary human T cells with ACHN cells. Left panels depict an enhanced view of an EdU + CD45 + trogosome within the intracellular space of an ACHN cell. Right panels depict an enhanced view of an EdU + CD45 + T cell for comparison purposes. (E) Representative max intensity projection images of trogosomes in three RCC cell lines generated from Z-stacks (top to bottom, A498, 786O, ACHN). Boxes in the merged images of middle and bottom panels indicate the region being enhanced in the split color panels to the right of the merged image. Arrows in the merged zoom panels indicate the location of a trogosome. All images taken on a Zeiss <t>710</t> laser scanning confocal, <t>40X</t> (NA 1.30).
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Image Search Results


(A) Flow cytometry results of Caki-1 cells that were cocultured with GFP-histone H2B primary T cells. Data shows the percentage of GFP-H2B + Caki-1 cells in coculture relative to monocultured Caki-1 cells. (B) Mean fluorescent intensity of GFP-H2B expression of cocultured Caki-1 cells compared to monoculture (p value = 0.0058, unpaired T test was used to analyze the data). (C) Flow cytometry analysis of RCC cell lines cocultured with EdU-labeled primary human T cells. Plots show the percentage of RCC cells that are EdU + and CD45 + post 16-hour coculture. (D) Max intensity projection of Z-stacked images taken from a 10:1 coculture of EdU-labeled primary human T cells with ACHN cells. Left panels depict an enhanced view of an EdU + CD45 + trogosome within the intracellular space of an ACHN cell. Right panels depict an enhanced view of an EdU + CD45 + T cell for comparison purposes. (E) Representative max intensity projection images of trogosomes in three RCC cell lines generated from Z-stacks (top to bottom, A498, 786O, ACHN). Boxes in the merged images of middle and bottom panels indicate the region being enhanced in the split color panels to the right of the merged image. Arrows in the merged zoom panels indicate the location of a trogosome. All images taken on a Zeiss 710 laser scanning confocal, 40X (NA 1.30).

Journal: PLOS One

Article Title: Renal cancer cells acquire immune surface protein through trogocytosis and horizontal gene transfer

doi: 10.1371/journal.pone.0325043

Figure Lengend Snippet: (A) Flow cytometry results of Caki-1 cells that were cocultured with GFP-histone H2B primary T cells. Data shows the percentage of GFP-H2B + Caki-1 cells in coculture relative to monocultured Caki-1 cells. (B) Mean fluorescent intensity of GFP-H2B expression of cocultured Caki-1 cells compared to monoculture (p value = 0.0058, unpaired T test was used to analyze the data). (C) Flow cytometry analysis of RCC cell lines cocultured with EdU-labeled primary human T cells. Plots show the percentage of RCC cells that are EdU + and CD45 + post 16-hour coculture. (D) Max intensity projection of Z-stacked images taken from a 10:1 coculture of EdU-labeled primary human T cells with ACHN cells. Left panels depict an enhanced view of an EdU + CD45 + trogosome within the intracellular space of an ACHN cell. Right panels depict an enhanced view of an EdU + CD45 + T cell for comparison purposes. (E) Representative max intensity projection images of trogosomes in three RCC cell lines generated from Z-stacks (top to bottom, A498, 786O, ACHN). Boxes in the merged images of middle and bottom panels indicate the region being enhanced in the split color panels to the right of the merged image. Arrows in the merged zoom panels indicate the location of a trogosome. All images taken on a Zeiss 710 laser scanning confocal, 40X (NA 1.30).

Article Snippet: and images were taken on a Zeiss 710 Laser Scanning Confocal microscope at 40X (NA = 1.30) or 63X (NA 1.40). images shown are max intensity projections of 60 Z-stacked images.

Techniques: Flow Cytometry, Expressing, Labeling, Comparison, Generated